ASTM D6974-2004 液体燃料中存活细菌和真菌计数的标准实施规范.过滤和培养过程
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【英文标准名称】:StandardPracticeforEnumerationofViableBacteriaandFungiinLiquidFuels&8212;FiltrationandCultureProcedures
【原文标准名称】:液体燃料中存活细菌和真菌计数的标准实施规范.过滤和培养过程
【标准号】:ASTMD6974-2004
【标准状态】:现行
【国别】:
【发布日期】:2004
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D02.14
【标准类型】:(Practice)
【标准水平】:()
【中文主题词】:细菌纲;培养;计数;过滤;菌类;液体燃料
【英文主题词】:bacteria;biodegradation;biodeterioration;colonyformingunit;enumeration;fuelmicrobiology;fungi;membranefiltertechnique;viablecount
【摘要】:Biodeteriogenicmicrobesinfectingfuelsystemstypicallyaremostabundantwithinslimeaccumulationsonsystemsurfacesoratthefuel-waterinterface(GuideD6469).However,itisoftenimpracticaltoobtainsamplesfromtheselocationswithinfuelsystems.Althoughthenumbersofviablebacteriaandfungirecoveredfromfuel-phasesamplesarelikelytobeseveralordersofmagnitudesmallerthanthosefoundinwater-phasesamples,fuel-phaseorganismsareoftenthemostreadilyavailableindicatorsoffuelandfuelsystemmicrobialcontamination.GrowthMediumSelectivity8212;GuideE1326discussesthelimitationsofgrowthmediumselection.Anymediumselectedwillfavorcolonyformationbysomespeciesandsuppresscolonyformationbyothers.Asnotedin6.3,physical,chemicalandphysiologicalvariablescanaffectviablecellenumerationtestresults.Sinceawiderangeofsamplesizes,ordilutionsthereof,canbeanalyzedbythemembranefiltertechnique(TestMethodsD5259andF1094),thetestsensitivitycanbeadjustedforthepopulationdensityexpectedinthesample.Enumerationdatashouldbeusedaspartofdiagnosticeffortsorroutineconditionmonitoringprograms.Enumerationdatashouldnotbeusedasfuelqualitycriteria.1.1Thispracticecoversamembranefilter(MF)procedureforthedetectionandenumerationofHeterotrophicbacteria(HPC)andfungiinliquidfuelswithkinematicviscosities8804;24mm2183;s-1atambienttemperature.1.2ThisquantitativepracticeisdrawnlargelyfromIPMethod385andTestMethodD5259.1.3Thistestmaybeperformedeitherinthefieldorinthelaboratory.1.4Theabilityofindividualmicrobestoformcoloniesonspecificgrowthmediadependsonthetaxonomyandphysiologicalstateofthemicrobestobeenumerated,thechemistryofthegrowthmedium,andincubationconditions.Consequently,testresultsshouldnotbeinterpretedasabsolutevalues.Rathertheyshouldbeusedaspartofadiagnosticorconditionmonitoringeffortthatincludesothertestparameters,inaccordancewithGuideD6469.1.5Thispracticeoffersalternativeoptionsfordeliveringfuelsamplemicrobestothefiltermembrane,volumesordilutionsfiltered,growthmediausedtocultivatefuel-bornemicrobes,andincubationtemperatures.Thisflexibilityisofferedtofacilitatediagnosticefforts.Whenthispracticeisusedaspartofaconditionmonitoringprogram,asingleprocedureshouldbeusedconsistently.1.6ThevaluesstatedinSIunitsaretoberegardedasthestandard.1.7Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:E31
【国际标准分类号】:75_160_20(LIQUIDFUELS)
【页数】:5P.;A4
【正文语种】:
【原文标准名称】:液体燃料中存活细菌和真菌计数的标准实施规范.过滤和培养过程
【标准号】:ASTMD6974-2004
【标准状态】:现行
【国别】:
【发布日期】:2004
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D02.14
【标准类型】:(Practice)
【标准水平】:()
【中文主题词】:细菌纲;培养;计数;过滤;菌类;液体燃料
【英文主题词】:bacteria;biodegradation;biodeterioration;colonyformingunit;enumeration;fuelmicrobiology;fungi;membranefiltertechnique;viablecount
【摘要】:Biodeteriogenicmicrobesinfectingfuelsystemstypicallyaremostabundantwithinslimeaccumulationsonsystemsurfacesoratthefuel-waterinterface(GuideD6469).However,itisoftenimpracticaltoobtainsamplesfromtheselocationswithinfuelsystems.Althoughthenumbersofviablebacteriaandfungirecoveredfromfuel-phasesamplesarelikelytobeseveralordersofmagnitudesmallerthanthosefoundinwater-phasesamples,fuel-phaseorganismsareoftenthemostreadilyavailableindicatorsoffuelandfuelsystemmicrobialcontamination.GrowthMediumSelectivity8212;GuideE1326discussesthelimitationsofgrowthmediumselection.Anymediumselectedwillfavorcolonyformationbysomespeciesandsuppresscolonyformationbyothers.Asnotedin6.3,physical,chemicalandphysiologicalvariablescanaffectviablecellenumerationtestresults.Sinceawiderangeofsamplesizes,ordilutionsthereof,canbeanalyzedbythemembranefiltertechnique(TestMethodsD5259andF1094),thetestsensitivitycanbeadjustedforthepopulationdensityexpectedinthesample.Enumerationdatashouldbeusedaspartofdiagnosticeffortsorroutineconditionmonitoringprograms.Enumerationdatashouldnotbeusedasfuelqualitycriteria.1.1Thispracticecoversamembranefilter(MF)procedureforthedetectionandenumerationofHeterotrophicbacteria(HPC)andfungiinliquidfuelswithkinematicviscosities8804;24mm2183;s-1atambienttemperature.1.2ThisquantitativepracticeisdrawnlargelyfromIPMethod385andTestMethodD5259.1.3Thistestmaybeperformedeitherinthefieldorinthelaboratory.1.4Theabilityofindividualmicrobestoformcoloniesonspecificgrowthmediadependsonthetaxonomyandphysiologicalstateofthemicrobestobeenumerated,thechemistryofthegrowthmedium,andincubationconditions.Consequently,testresultsshouldnotbeinterpretedasabsolutevalues.Rathertheyshouldbeusedaspartofadiagnosticorconditionmonitoringeffortthatincludesothertestparameters,inaccordancewithGuideD6469.1.5Thispracticeoffersalternativeoptionsfordeliveringfuelsamplemicrobestothefiltermembrane,volumesordilutionsfiltered,growthmediausedtocultivatefuel-bornemicrobes,andincubationtemperatures.Thisflexibilityisofferedtofacilitatediagnosticefforts.Whenthispracticeisusedaspartofaconditionmonitoringprogram,asingleprocedureshouldbeusedconsistently.1.6ThevaluesstatedinSIunitsaretoberegardedasthestandard.1.7Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:E31
【国际标准分类号】:75_160_20(LIQUIDFUELS)
【页数】:5P.;A4
【正文语种】:
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